Crystal Structure of the CSL-Notch-Mastermind Ternary Complex Bound to DNA

نویسندگان

  • Jeffrey J. Wilson
  • Rhett A. Kovall
چکیده

Notch signaling mediates communication between cells and is essential for proper embryonic patterning and development. CSL is a DNA binding transcription factor that regulates transcription of Notch target genes by interacting with coregulators. Transcriptional activation requires the displacement of corepressors from CSL by the intracellular portion of the receptor Notch (NotchIC) and the recruitment of the coactivator protein Mastermind to the complex. Here we report the 3.1 A structure of the ternary complex formed by CSL, NotchIC, and Mastermind bound to DNA. As expected, the RAM domain of Notch interacts with the beta trefoil domain of CSL; however, the C-terminal domain of CSL has an unanticipated central role in the interface formed with the Notch ankyrin repeats and Mastermind. Ternary complex formation induces a substantial conformational change within CSL, suggesting a molecular mechanism for the conversion of CSL from a repressor to an activator.

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Notch and MAML-1 Complexation Do Not Detectably Alter the DNA Binding Specificity of the Transcription Factor CSL

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Crystal structure of the nuclear effector of Notch signaling, CSL, bound to DNA.

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عنوان ژورنال:
  • Cell

دوره 124  شماره 

صفحات  -

تاریخ انتشار 2006